Mkrtchyan, H.; Scheler, S.; Klein, I.; Fahr, A.; Couraud, P. O.; Romero, I. A.; Weksler, B. and Liehr, T.
(2009).
![]() |
(Version of Record)
Due to publisher licensing restrictions, this file is not available for public download Click here to request a copy from the OU Author. |
DOI (Digital Object Identifier) Link: | https://doi.org/10.1159/000253080 |
---|---|
Google Scholar: | Look up in Google Scholar |
Abstract
The immortalized human cerebral microvessel endothelial cell line hCMEC/D3 has been repeatedly used as a model of human blood-brain barrier (BBB). hCMEC/D3 cells between passage 25 and 35 are most often applied in research, remained phenotypically nontransformed, and cells maintained many characteristics of human brain endothelial cells. Also hCMEC/D3 was thought to have conserved a normal diploid karyotype over all these passages. Here we characterized the cell line using high-resolution multicolor fluorescence in situ hybridization (FISH) approaches and revealed a complex karyotype in the 30th passage. Clonal cryptic unbalanced structural rearrangements and numerical aberrations were discovered and described as follows: 45~48,XX, 'X,del(5)(q11)[2],del(9)(q11)[3],+9[3],del(11)(q13~14)[2], der(14)t(14;21)(q32.33;q22.3)[28],der(15)t(9;15)(p11;p11)[13], dup(15)(p11q11)[5],der(21)t(17;21)(p12;q22)[9],�22[6][cp28]. In summary, a complex karyotype with clonal unbalanced chromosomal rearrangements is present in hCMEC/D3. Thus, we solicit to include molecular cytogenetics in the testing of all cell lines prior to application of their use in complex studies.
Item Type: | Journal Item |
---|---|
Copyright Holders: | 2009 S. Karger AG, Basel |
ISSN: | 1424-8581 |
Keywords: | hCMEC/D3; Karyotype; Microvessel; endothelial cell line; Multicolor banding (MCB) |
Academic Unit/School: | Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences Faculty of Science, Technology, Engineering and Mathematics (STEM) |
Item ID: | 23846 |
Depositing User: | Astrid Peterkin |
Date Deposited: | 20 Oct 2010 11:32 |
Last Modified: | 19 Dec 2017 09:37 |
URI: | http://oro.open.ac.uk/id/eprint/23846 |
Share this page: | ![]() ![]() ![]() ![]() |
Metrics
Altmetrics from Altmetric | Citations from Dimensions |