Mkrtchyan, H.; Scheler, S.; Klein, I.; Fahr, A.; Couraud, P. O.; Romero, I. A.; Weksler, B. and Liehr, T.
(2009).
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| DOI (Digital Object Identifier) Link: | http://dx.doi.org/10.1159/000253080 |
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Abstract
The immortalized human cerebral microvessel endothelial cell line hCMEC/D3 has been repeatedly used as a model of human blood-brain barrier (BBB). hCMEC/D3 cells between passage 25 and 35 are most often applied in research, remained phenotypically nontransformed, and cells maintained many characteristics of human brain endothelial cells. Also hCMEC/D3 was thought to have conserved a normal diploid karyotype over all these passages. Here we characterized the cell line using high-resolution multicolor fluorescence in situ hybridization (FISH) approaches and revealed a complex karyotype in the 30th passage. Clonal cryptic unbalanced structural rearrangements and numerical aberrations were discovered and described as follows: 45~48,XX, 'X,del(5)(q11)[2],del(9)(q11)[3],+9[3],del(11)(q13~14)[2], der(14)t(14;21)(q32.33;q22.3)[28],der(15)t(9;15)(p11;p11)[13], dup(15)(p11q11)[5],der(21)t(17;21)(p12;q22)[9],�22[6][cp28]. In summary, a complex karyotype with clonal unbalanced chromosomal rearrangements is present in hCMEC/D3. Thus, we solicit to include molecular cytogenetics in the testing of all cell lines prior to application of their use in complex studies.
| Item Type: | Journal Article |
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| Copyright Holders: | 2009 S. Karger AG, Basel |
| ISSN: | 1424-8581 |
| Keywords: | hCMEC/D3; Karyotype; Microvessel; endothelial cell line; Multicolor banding (MCB) |
| Academic Unit/Department: | Science > Life, Health and Chemical Sciences |
| Interdisciplinary Research Centre: | Biomedical Research Network (BRN) |
| Item ID: | 23846 |
| Depositing User: | Astrid Peterkin |
| Date Deposited: | 20 Oct 2010 11:32 |
| Last Modified: | 17 Mar 2014 19:43 |
| URI: | http://oro.open.ac.uk/id/eprint/23846 |
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