Mkrtchyan, H.; Scheler, S.; Klein, I.; Fahr, A.; Couraud, P. O.; Romero, I. A.; Weksler, B. and Liehr, T.
Molecular cytogenetic characterization of the human cerebral microvessel endothelial cell line hCMEC/D3.
Cytogenetic and Genome Research, 126 pp. 313–317.
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The immortalized human cerebral microvessel endothelial cell line hCMEC/D3 has been repeatedly used as a model of human blood-brain barrier (BBB). hCMEC/D3 cells between passage 25 and 35 are most often applied in research, remained phenotypically nontransformed, and cells maintained many characteristics of human brain endothelial cells. Also hCMEC/D3 was thought to have conserved a normal diploid karyotype over all these passages. Here we characterized the cell line using high-resolution multicolor fluorescence in situ hybridization (FISH) approaches and revealed a complex karyotype in the 30th passage. Clonal cryptic unbalanced structural rearrangements and numerical aberrations were discovered and described as follows: 45~48,XX, 'X,del(5)(q11),del(9)(q11),+9,del(11)(q13~14), der(14)t(14;21)(q32.33;q22.3),der(15)t(9;15)(p11;p11), dup(15)(p11q11),der(21)t(17;21)(p12;q22),ï¿½22[cp28]. In summary, a complex karyotype with clonal unbalanced chromosomal rearrangements is present in hCMEC/D3. Thus, we solicit to include molecular cytogenetics in the testing of all cell lines prior to application of their use in complex studies.
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