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Molecular cytogenetic characterization of the human cerebral microvessel endothelial cell line hCMEC/D3

Mkrtchyan, H.; Scheler, S.; Klein, I.; Fahr, A.; Couraud, P. O.; Romero, I. A.; Weksler, B. and Liehr, T. (2009). Molecular cytogenetic characterization of the human cerebral microvessel endothelial cell line hCMEC/D3. Cytogenetic and Genome Research, 126 pp. 313–317.

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The immortalized human cerebral microvessel endothelial cell line hCMEC/D3 has been repeatedly used as a model of human blood-brain barrier (BBB). hCMEC/D3 cells between passage 25 and 35 are most often applied in research, remained phenotypically nontransformed, and cells maintained many characteristics of human brain endothelial cells. Also hCMEC/D3 was thought to have conserved a normal diploid karyotype over all these passages. Here we characterized the cell line using high-resolution multicolor fluorescence in situ hybridization (FISH) approaches and revealed a complex karyotype in the 30th passage. Clonal cryptic unbalanced structural rearrangements and numerical aberrations were discovered and described as follows: 45~48,XX, 'X,del(5)(q11)[2],del(9)(q11)[3],+9[3],del(11)(q13~14)[2], der(14)t(14;21)(q32.33;q22.3)[28],der(15)t(9;15)(p11;p11)[13], dup(15)(p11q11)[5],der(21)t(17;21)(p12;q22)[9],�22[6][cp28]. In summary, a complex karyotype with clonal unbalanced chromosomal rearrangements is present in hCMEC/D3. Thus, we solicit to include molecular cytogenetics in the testing of all cell lines prior to application of their use in complex studies.

Item Type: Journal Article
Copyright Holders: 2009 S. Karger AG, Basel
ISSN: 1424-8581
Keywords: hCMEC/D3; Karyotype; Microvessel; endothelial cell line; Multicolor banding (MCB)
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 23846
Depositing User: Astrid Peterkin
Date Deposited: 20 Oct 2010 11:32
Last Modified: 25 Feb 2016 14:30
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