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Expression of cellular prion protein in the frontal and occipital lobe in Alzheimer's disease, diffuse Lewy Body disease, and in normal brain: an immunohistochemical study

Rezaie, Payam; Pontikis, Charlie C.; Hudson, Lance; Cairns, Nigel J. and Lantos, Peter L. (2005). Expression of cellular prion protein in the frontal and occipital lobe in Alzheimer's disease, diffuse Lewy Body disease, and in normal brain: an immunohistochemical study. Journal of Histochemistry and Cytochemistry, 53(8) pp. 929–940.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1369/jhc.4A6551.2005
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Abstract

Cellular prion protein (PrP(c)) is a glycoprotein expressed at low to moderate levels within the nervous system. Recent studies suggest that PrP(c) may possess neuroprotective functions and that its expression is upregulated in certain neurodegenerative disorders. We investigated whether PrP(c) expression is altered in the frontal and occipital cortex in two well-characterized neurodegenerative disorders--Alzheimer's disease (AD) and diffuse Lewy body disease (DLBD)--compared with that in normal human brain using immunohistochemistry and computerized image analysis. The distribution of PrP(c) was further tested for correlation with glial reactivity. We found that PrP(c) was localized mainly in the gray matter (predominantly in neurons) and expressed at higher levels within the occipital cortex in the normal human brain. Image analysis revealed no significant variability in PrP(c) expression between DLBD and control cases. However, blood vessels within the white matter of DLBD cases showed immunoreactivity to PrP(c). By contrast, this protein was differentially expressed in the frontal and occipital cortex of AD cases; it was markedly overexpressed in the former and significantly reduced in the latter. Epitope specificity of antibodies appeared important when detecting PrP(c). The distribution of PrP(c) did not correlate with glial immunoreactivity. In conclusion, this study supports the proposal that regional changes in expression of PrP(c) may occur in certain neurodegenerative disorders such as AD, but not in other disorders such as DLBD.

Item Type: Journal Article
ISSN: 0022-1554
Keywords: PrPc; immunohistochemistry; neurodegenerative diseases; image analysis
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 2341
Depositing User: Payam Rezaie
Date Deposited: 14 Jun 2006
Last Modified: 10 Mar 2014 09:26
URI: http://oro.open.ac.uk/id/eprint/2341
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