The Open UniversitySkip to content
 

Homing of stem cells to sites of inflammatory brain injury after intracerebral and intravenous administration: a longitudinal imaging study

Jackson, Johanna S.; Golding, Jon P.; Chapon, Catherine; Jones, William A. and Bhakoo, Kishore K. (2010). Homing of stem cells to sites of inflammatory brain injury after intracerebral and intravenous administration: a longitudinal imaging study. Stem Cells Research & Therapy, 1(2) pp. 1–17.

Warning

This is the latest version of this eprint.

Full text available as:
[img]
Preview
PDF (Version of Record) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (989Kb)
DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1186/scrt17
Google Scholar: Look up in Google Scholar

Abstract

Introduction

This study aimed to determine the homing potential and fate of epidermal neural crest stem cells (eNCSCs) derived from hair follicles, and bone marrow-derived stem cells (BMSCs) of mesenchymal origin, in a lipopolysaccharide (LPS)-induced inflammatory lesion model in the rat brain. Both eNCSCs and BMSCs are easily accessible from adult tissues by using minimally invasive procedures and can differentiate into a variety of neuroglial lineages. Thus, these cells have the potential to be used in autologous cell-replacement therapies, minimizing immune rejection, and engineered to secrete a variety of molecules.

Methods

Both eNCSCs and BMSCs were prelabeled with iron-oxide nanoparticles (IO-TAT-FITC) and implanted either onto the corpus callosum in healthy or LPS-lesioned animals or intravenously into lesioned animals. Both cell types were tracked longitudinally in vivo by using magnetic resonance imaging (MRI) for up to 30 days and confirmed by postmortem immunohistochemistry.

Results

Transplanted cells in nonlesioned animals remained localized along the corpus callosum. Cells implanted distally from an LPS lesion (either intracerebrally or intravenously) migrated only toward the lesion, as seen by the localized MRI signal void. Fluorescence microscopy of the FITC tag on the nanoparticles confirmed the in vivo MRI data,

Conclusions

This study demonstrated that both cell types can be tracked in vivo by using noninvasive MRI and have pathotropic properties toward an inflammatory lesion in the brain. As these cells differentiate into the glial phenotype and are derived from adult tissues, they offer a viable alternative autologous stem cell source and gene-targeting potential for neurodegenerative and demyelinating pathologies.

Item Type: Journal Article
Copyright Holders: 2010 The Authors
ISSN: 1757-6512
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetNot SetMRC
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Other Departments > Other Departments
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Related URLs:
Item ID: 22030
Depositing User: Jon Golding
Date Deposited: 13 Jul 2010 15:48
Last Modified: 24 Jul 2014 09:28
URI: http://oro.open.ac.uk/id/eprint/22030
Share this page:

Available Versions of this Item

  • Homing of stem cells to sites of inflammatory brain injury after intracerebral and intravenous administration: a longitudinal imaging study. (deposited 13 Jul 2010 15:48) [Currently Displayed]

Actions (login may be required)

View Item
Report issue / request change

Policies | Disclaimer

© The Open University   + 44 (0)870 333 4340   general-enquiries@open.ac.uk