Apolipoprotein D synthesis progressively increases in frontal cortex during human lifespan

Navarro, Ana; Del Valle, Eva; Juarez, Amalia; Martinez, Eva; Ordoñez, Cristina; Astudillo, Aurora and Tolivia, Jorge (2010). Apolipoprotein D synthesis progressively increases in frontal cortex during human lifespan. Age, 32(1) pp. 85–96.

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DOI (Digital Object Identifier) Link:	http://dx.doi.org/doi:10.1007/s11357-009-9117-0
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Abstract

Apolipoprotein D (apo D) is a lipocalin present in the nervous system that may be related to processes of reinnervation, regeneration and neuronal cell protection. In the other way, apo D expression has been correlated, in some brain regions, with normal aging and neurodegenerative diseases. To elucidate the regional and cellular expression of apo D in normal human brain during aging, we performed a detailed and extensive study in samples of post-mortem human cerebral cortices. To achieve this study, slot blot techniques, for protein and mRNA, as well as immunohistochemistry and hybridohistochemistry methods were used. A positive correlation for apo D expression with aging was found; furthermore, mRNA levels, as well as the protein ones, were higher in the white than in the grey matter. Immunohistochemistry and non-isotopic HIS showed that apo D is synthesized in both neurons and glial cells. Apo D expression is notorious in oligodendrocytes but with aging the number of neurons that synthesize apo D is increased. Our results indicate that apo D could play a fundamental role in central nervous system aging and in the reduction of products derivated from lipid peroxidation. The increment in the expression of apo D with aging can be included in a global mechanism of cellular protection to prevent the deleterious effects caused by aging.

Item Type:	Journal Article
Copyright Holders:	2010 American Aging Association
ISSN:	0161-9152
Funders:	MEC and FEDER (SAF2007-64076)
Extra Information:	The original publication is available at www.springerlink.com.
Keywords:	apolipoprotein D; aging; human; frontal cortex; in situ hybridization; immunohistochemistry
Academic Unit/Department:	Science > Life, Health and Chemical Sciences
Item ID:	18578
Depositing User:	Eva Del Valle Suarez
Date Deposited:	15 Dec 2009 16:48
Last Modified:	01 Nov 2012 12:02
URI:	http://oro.open.ac.uk/id/eprint/18578

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