Apolipoprotein D synthesis progressively increases in frontal cortex during human lifespan

Navarro, Ana; Del Valle, Eva; Juarez, Amalia; Martinez, Eva; Ordoñez, Cristina; Astudillo, Aurora and Tolivia, Jorge (2010). Apolipoprotein D synthesis progressively increases in frontal cortex during human lifespan. Age, 32(1) pp. 85–96.

Full text available as:

Preview

PDF (Accepted Manuscript) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (6Mb)

DOI (Digital Object Identifier) Link:	http://dx.doi.org/10.1007/s11357-009-9117-0

Google Scholar:	Look up in Google Scholar

Abstract

Apolipoprotein D (apo D) is a lipocalin present in the nervous system that may be related to processes of reinnervation, regeneration and neuronal cell protection. In the other way, apo D expression has been correlated, in some brain regions, with normal aging and neurodegenerative diseases. To elucidate the regional and cellular expression of apo D in normal human brain during aging, we performed a detailed and extensive study in samples of post-mortem human cerebral cortices. To achieve this study, slot blot techniques, for protein and mRNA, as well as immunohistochemistry and hybridohistochemistry methods were used. A positive correlation for apo D expression with aging was found; furthermore, mRNA levels, as well as the protein ones, were higher in the white than in the grey matter. Immunohistochemistry and non-isotopic HIS showed that apo D is synthesized in both neurons and glial cells. Apo D expression is notorious in oligodendrocytes but with aging the number of neurons that synthesize apo D is increased. Our results indicate that apo D could play a fundamental role in central nervous system aging and in the reduction of products derivated from lipid peroxidation. The increment in the expression of apo D with aging can be included in a global mechanism of cellular protection to prevent the deleterious effects caused by aging.

Item Type:

Journal Article

2010 American Aging Association

ISSN:

0161-9152

External Project Funding Details:

Funded Project Name	Project ID	Funding Body
Not Set	Not Set	MEC and FEDER (SAF2007-64076)

Extra Information:

The original publication is available at www.springerlink.com.

Keywords:

apolipoprotein D; aging; human; frontal cortex; in situ hybridization; immunohistochemistry

Academic Unit/Department:

Science > Life, Health and Chemical Sciences

Item ID:

18578

Depositing User:

Eva Del Valle Suarez

Date Deposited:

15 Dec 2009 16:48

Last Modified:

01 Nov 2012 12:02

URI:

http://oro.open.ac.uk/id/eprint/18578

Actions (login may be required)

	View Item
	Report issue / request change

Policies | Disclaimer