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Expression and induction of p-glycoprotein-1 on cultured human brain endothelium

Male, David K. (2009). Expression and induction of p-glycoprotein-1 on cultured human brain endothelium. Journal of Cerebral Blood Flow and Metabolism, 29(11) pp. 1760–1763.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1038/jcbfm.2009.101
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Abstract

The ABC-transporter, p-glycoprotein-1 (pgp-1), is expressed on brain endothelium and is reported to be induced by several cytotoxic drugs, which are themselves substrates of pgp-1. Pgp-1 was increased on a human brain endothelial cell line (hCMEC/D3) after treatment with puromycin or verapamil. However, flow cytometry showed that the apparent upregulation caused by puromycin was not because of a global increase in expression levels, but selective cell death of a subpopulation of endothelium expressing the lowest levels of pgp-1. If a cytotoxic substrate of pgp-1 increases pgp-1 expression in vitro, it can easily be misinterpreted as a transcriptional activator of pgp-1.

Item Type: Journal Article
Copyright Holders: 2009 International Society for Cerebral Blood Flow & Metabolism
ISSN: 0271-678X
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Innovation, Knowledge & Development research centre (IKD)
Biomedical Research Network (BRN)
Item ID: 18023
Depositing User: David Male
Date Deposited: 23 Sep 2009 15:55
Last Modified: 07 Mar 2014 13:39
URI: http://oro.open.ac.uk/id/eprint/18023
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