Male, David K.
|DOI (Digital Object Identifier) Link:||http://dx.doi.org/10.1038/jcbfm.2009.101|
|Google Scholar:||Look up in Google Scholar|
The ABC-transporter, p-glycoprotein-1 (pgp-1), is expressed on brain endothelium and is reported to be induced by several cytotoxic drugs, which are themselves substrates of pgp-1. Pgp-1 was increased on a human brain endothelial cell line (hCMEC/D3) after treatment with puromycin or verapamil. However, flow cytometry showed that the apparent upregulation caused by puromycin was not because of a global increase in expression levels, but selective cell death of a subpopulation of endothelium expressing the lowest levels of pgp-1. If a cytotoxic substrate of pgp-1 increases pgp-1 expression in vitro, it can easily be misinterpreted as a transcriptional activator of pgp-1.
|Item Type:||Journal Article|
|Copyright Holders:||2009 International Society for Cerebral Blood Flow & Metabolism|
|Academic Unit/Department:||Science > Life, Health and Chemical Sciences|
|Interdisciplinary Research Centre:||Innovation, Knowledge & Development research centre (IKD)
Biomedical Research Network (BRN)
|Depositing User:||David Male|
|Date Deposited:||23 Sep 2009 15:55|
|Last Modified:||07 Mar 2014 13:39|
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