The Open UniversitySkip to content
 

Methamphetamine disrupts blood–brain barrier function by induction of oxidative stress in brain endothelial cells

Ramirez, Servio H.; Potula, Raghava; Fan, Shongshan; Eidem, Tess; Papugani, Anil; Reichenbach, Nancy; Dykstra, Holly; Weksler, Babette B.; Romero, Ignacio A.; Couraud, Pierre O. and Persidsky, Yuri (2009). Methamphetamine disrupts blood–brain barrier function by induction of oxidative stress in brain endothelial cells. Journal of Cerebral Blood Flow and Metabolism, 29(12) pp. 1933–1945.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1038/jcbfm.2009.112
Google Scholar: Look up in Google Scholar

Abstract

Methamphetamine (METH), a potent stimulant with strong euphoric properties, has a high abuse liability and long-lasting neurotoxic effects. Recent studies in animal models have indicated that METH can induce impairment of the blood-brain barrier (BBB), thus suggesting that some of the neurotoxic effects resulting from METH abuse could be the outcome of barrier disruption. In this study, we provide evidence that METH alters BBB function through direct effects on endothelial cells and explore possible underlying mechanisms leading to endothelial injury. We report that METH increases BBB permeability in vivo, and exposure of primary human brain microvascular endothelial cells (BMVEC) to METH diminishes the tightness of BMVEC monolayers in a dose- and time-dependent manner by decreasing the expression of cell membrane-associated tight junction (TJ) proteins. These changes were accompanied by the enhanced production of reactive oxygen species, increased monocyte migration across METH-treated endothelial monolayers, and activation of myosin light chain kinase (MLCK) in BMVEC. Antioxidant treatment attenuated or completely reversed all tested aspects of METH-induced BBB dysfunction. Our data suggest that BBB injury is caused by METH-mediated oxidative stress, which activates MLCK and negatively affects the TJ complex. These observations provide a basis for antioxidant protection against brain endothelial injury caused by METH exposure.

Item Type: Journal Article
Copyright Holders: 2009 International Society for Cerebral Blood Flow & Metabolism
ISSN: 0271-678X
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 17889
Depositing User: Users 9108 not found.
Date Deposited: 11 Aug 2009 12:36
Last Modified: 07 Mar 2014 15:06
URI: http://oro.open.ac.uk/id/eprint/17889
Share this page:

Altmetrics

Scopus Citations

Actions (login may be required)

View Item
Report issue / request change

Policies | Disclaimer

© The Open University   + 44 (0)870 333 4340   general-enquiries@open.ac.uk