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Microtubule interfering agents and KSP inhibitors induce the phosphorylation of the nuclear protein p54(nrb), an event linked to G2/M arrest

Casado, P.; Prado, M. A; Zuazua-Villar, P.; Del Valle, Eva; Artime, N.; Cabal-Hierro, L.; Ruperez, P.; Burlingame, A. L.; Lazo, P. S. and Ramos, S. (2009). Microtubule interfering agents and KSP inhibitors induce the phosphorylation of the nuclear protein p54(nrb), an event linked to G2/M arrest. Journal of Proteomics, 71(6) pp. 592–600.

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DOI (Digital Object Identifier) Link: https://doi.org/10.1016/j.jprot.2008.09.001
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Abstract

Microtubule interfering agents (MIAs) are anti-tumor drugs that inhibit microtubule dynamics, while kinesin spindle protein (KSP) inhibitors are substances that block the formation of the bipolar spindle during mitosis. All these compounds cause G2/M arrest and cell death. Using 2D-PAGE followed by Nano-LC-ESI-Q-ToF analysis, we found that MIAs such as vincristine (Oncovin) or paclitaxel (Taxol) and KSP inhibitors such as S-tritil-l-cysteine induce the phosphorylation of the nuclear protein p54(nrb) in HeLa cells. Furthermore, we demonstrate that cisplatin (Platinol), an anti-tumor drug that does not cause M arrest, does not induce this modification. We show that the G2/M arrest induced by MIAs is required for p54(nrb) phosphorylation. Finally, we demonstrate that CDK activity is required for MIA-induced phosphorylation of p54(nrb).

Item Type: Journal Article
Copyright Holders: 2009 Unknown
ISSN: 1874-3919
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetNot SetFondo de Investigación de la Seguridad Social (FISS-05-PI-042664)
Not SetNot SetMinisterio de Educación y Ciencia (SAF2006-09686)
Not SetNot SetFICYT
Academic Unit/Department: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 16350
Depositing User: Eva Del Valle Suarez
Date Deposited: 21 May 2009 16:01
Last Modified: 05 Oct 2016 00:06
URI: http://oro.open.ac.uk/id/eprint/16350
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