Casado, P.; Prado, M. A; Zuazua-Villar, P.; Del Valle, Eva; Artime, N.; Cabal-Hierro, L.; Ruperez, P.; Burlingame, A. L.; Lazo, P. S. and Ramos, S.
(2009).
Microtubule interfering agents and KSP inhibitors induce the phosphorylation of the nuclear protein p54(nrb), an event linked to G2/M arrest.
Journal of Proteomics, 71(6),
pp. 592–600.
Full text available as:
Abstract
Microtubule interfering agents (MIAs) are anti-tumor drugs that inhibit microtubule dynamics, while kinesin spindle protein (KSP) inhibitors are substances that block the formation of the bipolar spindle during mitosis. All these compounds cause G2/M arrest and cell death. Using 2D-PAGE followed by Nano-LC-ESI-Q-ToF analysis, we found that MIAs such as vincristine (Oncovin) or paclitaxel (Taxol) and KSP inhibitors such as S-tritil-l-cysteine induce the phosphorylation of the nuclear protein p54(nrb) in HeLa cells. Furthermore, we demonstrate that cisplatin (Platinol), an anti-tumor drug that does not cause M arrest, does not induce this modification. We show that the G2/M arrest induced by MIAs is required for p54(nrb) phosphorylation. Finally, we demonstrate that CDK activity is required for MIA-induced phosphorylation of p54(nrb).
| Item Type: |
Journal Article
|
| Copyright Holders: |
2009 Unknown |
| ISSN: |
1874-3919 |
| Funders: |
Fondo de Investigación de la Seguridad Social (FISS-05-PI-042664), Ministerio de Educación y Ciencia (SAF2006-09686), FICYT |
| Academic Unit/Department: |
Science > Life, Health and Chemical Sciences |
| Item ID: |
16350 |
| Depositing User: |
Eva Del Valle Suarez
|
| Date Deposited: |
21 May 2009 16:01 |
| Last Modified: |
28 Oct 2012 13:21 |
| URI: |
http://oro.open.ac.uk/id/eprint/16350 |
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