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Three-dimensional adipocyte cultures and co-cultures to study tissue interactions

Daya, Sandeep; Loughlin, Jane and MacQueen, Hilary (2009). Three-dimensional adipocyte cultures and co-cultures to study tissue interactions. In: NC3Rs/BBSRC Symposium Tissue Engineering: a new dimension to animal replacement, 2-3 April 2009, Royal College of Surgeons, London UK.

URL: http://www.nc3rs.org.uk/event.asp?id=987
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Abstract

Adipocytes play many important roles within the body, though until recently their importance has frequently gone unrecognised. They are well known for their activities in fat metabolism and storage, but they are also significant secretory cells, and play roles in modulating immune function and provisioning stem cell development. Different adipose depots are specialised to interact in different ways with neighbouring cells, tissues and organs, and there is now an emerging body of evidence demonstrating their roles in areas as diverse as gut function, immune function and cardiac function.

Adipocytes are difficult to study in conventional culture: their buoyant density is such that they float, so are easily lost during medium changes, and their large size makes them too fragile to withstand many routine manipulations. When they lyse they release quantities of triacylglycerols which interfere with many biochemical analyses. Thus much of the work on adipocytes has had to be carried out in vivo.

We have developed a 3D culture system in which preadipocytes are differentiated to functional, mature adipocytes which retain many of the properties of ex vivo adipocytes. The adipocytes remain in place during medium changes, and their secretory activities can be monitored by assaying culture supernatants. Moreover, the culture conditions are mild, and flexible enough to accommodate co-cultures with cells requiring different culture conditions. It is therefore an ideal system for studying the interactions between adipocytes and other cell types that underpin tissue and organ function.

Item Type: Conference Item
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetNot SetBBSRC (Biotechnology and Biological Sciences Research Council)
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 16231
Depositing User: Hilary MacQueen
Date Deposited: 19 Jun 2009 09:13
Last Modified: 07 Mar 2014 13:43
URI: http://oro.open.ac.uk/id/eprint/16231
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