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Passive avoidance training is correlated with decreased cell proliferation in the chick hippocampus

Nikolakopoulou, A. M; Dermon, C. R.; Panagis, L.; Pavlidis, M. and Stewart, M. G. (2006). Passive avoidance training is correlated with decreased cell proliferation in the chick hippocampus. European Journal of Neuroscience, 24(9) pp. 2631–2642.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1111/j.1460-9568.2006.05133.x
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Abstract

One-trial passive avoidance learning (PAL), where the aversive stimulus is the bitter-tasting substance methylanthranilate (MeA), affects neuronal and synaptic plasticity in learning-related areas of day-old domestic chicks (Gallus domesticus). Here, cell proliferation was examined in the chick forebrain by using 5-bromo-2-deoxyuridine (BrdU) at 24 h and 9 days after PAL. At 24 h post-BrdU injection, there was a significant reduction in labelling in MeA-trained chicks in both the dorsal hippocampus and area parahippocampalis, in comparison to controls. Moreover, double-immunofluorescence labelling for BrdU and the nuclear neuronal marker (NeuN) showed a reduction of neuronal cells in the dorsal hippocampus of the MeA-trained group compared with controls (35 and 49%, respectively). There was no difference in BrdU labelling in hippocampal regions between trained and control groups of chicks at 9 days post-BrdU injection; however, the number of BrdU-labelled cells was considerably lower than at 24 h post-BrdU injection, possibly due to migration of cells within the telencephalon rather than cell loss as apoptotic analyses at 24 h and 9 days post-BrdU injection did not demonstrate differences in cell death between treatment groups. Cortisol levels increased in the chick hippocampus of MeA-trained birds 20 min after PAL, suggesting the possibility of a stress-related mechanism of cell proliferation reduction in the hippocampus. In contrast to hippocampal areas, the olfactory bulb, an area strongly stimulated by the strong-smelling MeA, showed increased cell genesis in comparison to controls at both 24 h and 9 days post-training.

Item Type: Journal Article
ISSN: 0953-816X
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 16064
Depositing User: Users 8955 not found.
Date Deposited: 07 May 2009 10:33
Last Modified: 07 Mar 2014 15:05
URI: http://oro.open.ac.uk/id/eprint/16064
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