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P-glycoprotein and breast cancer resistance protein restrict apical-to-basolateral permeability of human brain endothelium to amyloid-β

Tai, Leon M.; Loughlin, A. Jane; Male, David K. and Romero, Ignacio A. (2009). P-glycoprotein and breast cancer resistance protein restrict apical-to-basolateral permeability of human brain endothelium to amyloid-β. Journal of Cerebral Blood Flow and Metabolism, 29(6) pp. 1079–1083.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1038/jcbfm.2009.42
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Abstract

The clearance of amyloid beta (Aβ) from the brain represents a novel therapeutic target for Alzheimer’s disease. Conflicting data exists as to the contribution of ATP binding cassette transporters to the clearance of Aβ through the blood-brain barrier. We therefore investigated whether Aβ could be a substrate for P-glycoprotein (P-gp) and/or breast cancer resistance protein (BCRP) using a human brain endothelial cell line, hCMEC/D3. P-gp and BCRP inhibition increased apical-to-basolateral, but not basolateral-to-apical, permeability of hCMEC/D3 cells to 125I Aβ 1-40. Our in vitro data suggest that P-gp and BCRP might act to prevent blood-borne Aβ 1-40 entering the brain.

Item Type: Journal Article
Copyright Holders: 2009 ISCBFM
ISSN: 0271-678X
Keywords: Alzheimer’s disease; amyloid beta; BCRP; blood–brain barrier; P-gp
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Innovation, Knowledge & Development research centre (IKD)
Item ID: 15523
Depositing User: Astrid Peterkin
Date Deposited: 17 Apr 2009 09:16
Last Modified: 07 Mar 2014 13:39
URI: http://oro.open.ac.uk/id/eprint/15523
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