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Expression of ADAM-17, TIMP-3 and fractalkine in the human adult brain endothelial cell line, hCMEC/D3, following pro-inflammatory cytokine treatment

Hurst, Louise A.; Bunning, Rowena A.D.; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette B.; Sharrack, Basil and Woodroofe, M. Nicola (2009). Expression of ADAM-17, TIMP-3 and fractalkine in the human adult brain endothelial cell line, hCMEC/D3, following pro-inflammatory cytokine treatment. Journal of Neuroimmunology, 210(1-2) pp. 108–112.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1016/j.jneuroim.2009.02.008
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Abstract

ADAM-17 expression is localised to endothelial cells in the human central nervous system (CNS) and is increased in multiple sclerosis (MS) white matter, suggesting a role in MS pathogenesis. Expression of ADAM-17, TIMP-3, and fractalkine were investigated in a human brain endothelial cell line (hCMEC/D3) after pro-inflammatory cytokine treatment. Tumour necrosis factor (TNF) significantly increased fractalkine mRNA (>100 fold) and protein expression, which was associated with increased shedding of fractalkine from the cell. Fractalkine shedding may regulate immune cell trafficking into the CNS, however, this does not appear to be directly controlled by ADAM-17 activity.

Item Type: Journal Article
Copyright Holders: 2009 Elsevier B.V.
ISSN: 0165-5728
Keywords: ADAM-17; Multiple sclerosis; Fractalkine; Endothelial cells
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 15521
Depositing User: Astrid Peterkin
Date Deposited: 31 Mar 2009 15:03
Last Modified: 07 Mar 2014 15:06
URI: http://oro.open.ac.uk/id/eprint/15521
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