Lim, Joseph C.; Kania, Katarzyna D.; Wijesuriya, Hasini; Chawla, Sangeeta; Sethi, Jaswinder K.; Pulaski, Lukasz; Romero, Ignacio A.; Couraud, Pierre O.; Weksler, Babette B.; Hladky, Stephen B. and Barrand, Margery A.
|DOI (Digital Object Identifier) Link:||http://dx.doi.org/10.1111/j.1471-4159.2008.05537.x|
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This study investigates involvement of β-catenin signalling in regulation of p-glycoprotein (p-gp) expression in endothelial cells derived from brain vasculature. Pharmacological interventions that enhance or that block β-catenin signalling were applied to primary rat brain endothelial cells and to immortalized human brain endothelial cells, hCMEC/D3, nuclear translocation of β-catenin being determined by immunocytochemistry and by western blot analysis to confirm effectiveness of the manipulations. Using the specific glycogen synthase kinase-3 (GSK-3) inhibitor 6-bromoindirubin-3'-oxime enhanced β-catenin and increased p-gp expression including activating the MDR1 promoter. These increases were accompanied by increases in p-gp-mediated efflux capability as observed from alterations in intracellular fluorescent calcein accumulation detected by flow cytometry. Similar increases in p-gp expression were noted with other GSK-3 inhibitors, i.e. 1-azakenpaullone or LiCl. Application of Wnt agonist [2-amino-4-(3,4-(methylenedioxy) benzylamino)-6-(3-methoxyphenyl)pyrimidine] also enhanced β-catenin and increased transcript and protein levels of p-gp. By contrast, down-regulating the pathway using Dickkopf-1 or quercetin decreased p-gp expression. Similar changes were observed with multidrug resistance protein 4 and breast cancer resistance protein, both known to be present at the blood–brain barrier. These results suggest that regulation of p-gp and other multidrug efflux transporters in brain vasculature can be influenced by β-catenin signalling.
|Item Type:||Journal Article|
|Keywords:||β-catenin; breast cancer resistant protein; glycogen synthase kinase-3; multidrug resistance protein 4; p-glycoprotein; brain endothelial cells;|
|Academic Unit/Department:||Science > Life, Health and Chemical Sciences
|Interdisciplinary Research Centre:||Biomedical Research Network (BRN)|
|Depositing User:||Astrid Peterkin|
|Date Deposited:||28 Jul 2008 09:39|
|Last Modified:||14 Jan 2016 17:03|
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